Proso millet (Panicum miliaceum L.) is generally regarded as tolerant to various stress conditions; however, it has not been studied for variability in acid soil tolerance. A short-term bioassay was used to evaluate genotype-response to acid soil high in extractable and exchangeable aluminum (Al). Four genotypes showed susceptibility, six were intermediate, and four proved to be tolerant, indicating the potential for improving acid soil tolerance within this species.