RADIOENZYMATIC ASSAY FOR HISTAMINE - DEVELOPMENT AND VALIDATION

被引：5
|
作者：
HENRY, DP
BOWSHER, RR
VERBURG, KM
机构：
[1] INDIANA UNIV, SCH MED, DEPT BIOCHEM, INDIANAPOLIS, IN 46202 USA
[2] ELI LILLY & CO, LILLY LAB CLIN RES, INDIANAPOLIS, IN 46285 USA
[3] INDIANA UNIV, SCH MED, DEPT MED, INDIANAPOLIS, IN 46202 USA
[4] INDIANA UNIV, SCH MED, DEPT PHARMACOL, INDIANAPOLIS, IN 46202 USA
来源：
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY | 1991年 / 26卷
关键词：
GASTRIC SECRETION; HISTAMINE; HUMAN; RADIOENZYMATIC ASSAY;
D O I：
10.3109/00365529109093174
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
Radioenzymatic assays are sensitive analytic tools that use an enzyme to quantify a substrate for that enzyme. Purified histamine N-methyltransferase has been used as the basis for an assay for histamine. The sensitivity of the procedure is < 10 fmol. The specificity of the assay is increased when the transferase reaction is carried out at 0-3-degrees-C. Data documenting the precision of the assay, the stability of histamine in human plasma, and the gastric secretory rate of histamine are presented along with a chronologic description of the development of the technique.
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页码：26 / 31
页数：6
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