USE OF BARIUM SALTS OF PROTECTED DEOXYRIBONUCLEOSIDE-3' P-CHLOROPHENYL PHOSPHATES FOR CONSTRUCTION OF OLIGONUCLEOTIDES BY THE PHOSPHOTRIESTER METHOD - HIGH-YIELD SYNTHESIS OF DINUCLEOTIDE BLOCKS

被引：40

作者：

GOUGH, GR ^{[1
]}

COLLIER, KJ ^{[1
]}

WEITH, HL ^{[1
]}

GILHAM, PT ^{[1
]}

机构：

[1] PURDUE UNIV,DEPT BIOCHEM,W LAFAYETTE,IN 47907

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 7卷 / 07期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1093/nar/7.7.1955

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new experimental approach to the synthesis of polydeoxyribonucleotides via the phosphotriester method involves construction of oligonucleotide blocks by direct use of the easily prepared barium salts of O5′.N-protected deoxyribonucleoside-3′ p-chlorophenyl phosphates aa the key monomers in condensation reactions. The procedure has been demonstrated by the rapid synthesis in high yield and purity of all sixteen fully protected dinucleotides [(MeO)2Tr]dN′-dN′°.-(CNEt), (where dN′ · dT, dbzC, dbzA, or dibG; ° · diesterifled p-chlorophenyl phosphate). This set of molecules constitutes a syllabary" for the preparation of defined sequence oligonucleotides. © 1979 Information Retrieval Limited."

引用

页码：1955 / 1964

页数：10

共 7 条

[1] CHEMICAL SYNTHESIS OF A SELF-COMPLEMENTARY OCTANUCLEOTIDE, DG-G-T-T-A-A-C-C BY A MODIFIED TRIESTER METHOD [J].