DETECTION OF CROSS-REACTING IDIOTYPES IN SERA OF LYMPHOMA PATIENTS BY INVERSE MONOCLONAL RADIOIMMUNOASSAY

被引：5

作者：

IVANOVIC, V ^{[1
]}

POPOVIC, L ^{[1
]}

KOVACINA, K ^{[1
]}

DIMITRIJEVIC, L ^{[1
]}

ILIC, D ^{[1
]}

PIROZKOV, A ^{[1
]}

BALTIC, V ^{[1
]}

机构：

[1] INST ONCOL, SREMSKA KAMENICA, YUGOSLAVIA

来源：

ACTA HAEMATOLOGICA | 1990年 / 84卷 / 02期

关键词：

B cell lymphoma; Cross-reacting idiotype; Inverse monoclonal radioimmunoassay; Serum;

D O I：

10.1159/000205030

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

An anti-idiotypic IgGlK murine monoclonal antibody (MoAb) Y7 against purified monoclonal IgMiU, derived from a patient with Waldenstrom’s macroglobulinemia, has been generated. This antibody cross-reacted with the tumor-derived idiotypes of patients with B cell non Hodgkin’s lymphoma as measured by competitive inverse solid radioimmunoassay using unpurified serum samples. Our results with the inhibition curves of 10 sera of normal donors and 60 sera of lymphoma patients indicate that 21 lymphoma patients revealed cross-reactivities >7%, the mean value observed in normal donors. Of these, 5 sera cross-reacted strongly, in the range of 43-163%, revealing a frequency of positive cross-reactivity for MoAb Y7 of 1/12 sera of lymphoma patients. The generation of a panel of anti-idiotypic antibodies which cross-react with different tumor-derived Ig in serum may be valuable for monitoring the disease in a high proportion of NHL patients. © 1990 S. Karger AG, Basel.

引用

页码：64 / 67

页数：4

共 16 条

[1]

GIARDINA SL, 1985, BLOOD, V66, P1017

[2]

GIARDINA SL, 1985, J IMMUNOL, V135, P653

[3]

IVANOVIC V, 1987, RADIOL JUGOSL, V21, P19

[4]

KOVACINA K, 1988, RADIOL JUGOSL, V22, P261

[5]

MAYUMI M, 1982, J IMMUNOL, V129, P904

[6] A CLINICAL-TRIAL OF ANTI-IDIOTYPE THERAPY FOR B-CELL MALIGNANCY [J].

MEEKER, TC ;

LOWDER, J ;

MALONEY, DG ;

MILLER, RA ;

THIELEMANS, K ;

WARNKE, R ;

LEVY, R .

BLOOD, 1985, 65 (06) :1349-1363

[7] TREATMENT OF B-CELL LYMPHOMA WITH MONOCLONAL ANTI-IDIOTYPE ANTIBODY [J].

MILLER, RA ;

MALONEY, DG ;

WARNKE, R ;

LEVY, R .

NEW ENGLAND JOURNAL OF MEDICINE, 1982, 306 (09) :517-522

[8] PLATE BINDING ASSAY FOR MONOCLONAL ANTI-RECEPTOR ANTIBODIES [J].

MORGAN, DO ;

ROTH, RA .

ENDOCRINOLOGY, 1985, 116 (03) :1224-1226

[9]

RADL J, 1985, TOPICS AGING RES EUR, V5, P1

[10]

RANKIN EM, 1984, NEW ENGL J MED, V311, P470

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