An in vitro cultivation model for Salmonella having contact to epithelial cells was developed, which led to an increase in the production of toxic substances. The toxin assay on CHO-K1 cells was used for the determination of the toxic activities. Salmonella strains cultivated in contact with a monolayer of the intestinal cell line IEC-6 produced considerably more toxin than Salmonella strains cultivated on VERO cells. The toxin formed was heat-labile.
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Washington State Univ, Coll Vet Med, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA
Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Stockholm, SwedenWashington State Univ, Coll Vet Med, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA
Wrande, Marie
Andrews-Polymenis, Helene
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Texas A&M Univ, Dept Microbial Pathogenesis & Immunol, Coll Med, Bryan, TX USAWashington State Univ, Coll Vet Med, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA
Andrews-Polymenis, Helene
Twedt, Donna J.
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Washington State Univ, Coll Vet Med, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USAWashington State Univ, Coll Vet Med, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA
Twedt, Donna J.
Steele-Mortimer, Olivia
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NIAID, Bacteriol Lab, Rocky Mt Labs, NIH, Hamilton, MT USAWashington State Univ, Coll Vet Med, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA
Steele-Mortimer, Olivia
Porwollik, Steffen
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Univ Calif Irvine, Dept Microbiol & Mol Genet, Irvine, CA 92717 USAWashington State Univ, Coll Vet Med, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA
Porwollik, Steffen
McClelland, Michael
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Univ Calif Irvine, Dept Microbiol & Mol Genet, Irvine, CA 92717 USAWashington State Univ, Coll Vet Med, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA
McClelland, Michael
Knodler, Leigh A.
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Washington State Univ, Coll Vet Med, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USAWashington State Univ, Coll Vet Med, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA