OPTIMIZATION OF POLYETHYLENE-GLYCOL MEDIATED TRANSIENT GENE-EXPRESSION IN PEA PROTOPLASTS

被引：4

作者：

NICOLAISEN, M ^{[1
]}

POULSEN, GB ^{[1
]}

机构：

[1] DANISH INST PLANT & SOIL SCI, BIOTECHNOL GRP, LOTTENBORGVEJ 2, DK-2800 LYNGBY, DENMARK

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1993年 / 35卷 / 01期

关键词：

BETA-GLUCURONIDASE; PEA; POLYETHYLENE GLYCOL; TRANSIENT EXPRESSION;

D O I：

10.1007/BF00043945

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have investigated factors influencing polyethylene glycol mediated DNA uptake and beta-glucuronidase expression in pea (Pisum sativum L.) protoplasts. It was found that for optimal beta-glucuronidase expression the molecular weight and concentration of polyethylene glycol should be 4000 and 20%, respectively. The amount of plasmid DNA should be 25 mug per 5 x 10(5) protoplasts in each treatment, and the concentration of Mg2+ in the transformation buffer should be 15 mM. The optimized protocol was applicable to all four pea cultivars tested.

引用

页码：93 / 97

页数：5

共 24 条

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