KINESIN LIGHT-CHAIN ISOFORMS IN CAENORHABDITIS-ELEGANS

被引：26
作者：
FAN, J
AMOS, LA
机构：
[1] MRC Laboratory of Molecular Biology, Cambridge CB2 2QH, Hills Road
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1994年 / 240卷 / 05期
关键词：
MICROTUBULES; ALTERNATIVE SPLICING; PROTEIN SECONDARY STRUCTURE; UNC-116;
D O I：
10.1006/jmbi.1994.1465
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
cDNAs for two isoforms of kinesin light chain in Caenorhabditis elegans have been cloned and sequenced; the deduced M(r) values of the polypeptides are 60,338 and 58,938. Surprisingly, two independent cDNA libraries, each derived from worms at mixed stages of development, provided different single isoforms. The two isoforms are apparently derived from a single gene but have differences at both N and C termini of the predicted protein sequences. The sequences are highly homologous to those from other species, except for some minor features that may be related to the shortened form of the kinesin heavy chain, the product of gene unc-116, with which the light chains are assumed to associate. © 1994 Academic Press Limited.
引用
收藏
页码：507 / 512
页数：6
相关论文
共 36 条
[1]  
AMOS LA, 1987, J CELL SCI, V87, P105
[2]   SINGLE KINESIN MOLECULES CROSSBRIDGE MICROTUBULES IN-VITRO [J].
ANDREWS, SB ;
GALLANT, PE ;
LEAPMAN, RD ;
SCHNAPP, BJ ;
REESE, TS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (14) :6503-6507
[3]  
BARSTEAD RJ, 1989, J BIOL CHEM, V264, P10177
[4]  
BEUSHAUSEN S, 1993, DNA CELL BIOL, V10, P901
[5]   CLONING AND GENETIC-CHARACTERIZATION OF THE HUMAN KINESIN LIGHT-CHAIN (KLC) GENE [J].
CABEZAARVELAIZ, Y ;
SHIH, LCN ;
HARDMAN, N ;
ASSELBERGS, F ;
BILBE, G ;
SCHMITZ, A ;
WHITE, B ;
SICILIANO, MJ ;
LACHMAN, LB .
DNA AND CELL BIOLOGY, 1993, 12 (10) :881-892
[6]   YACS AND THE C-ELEGANS GENOME [J].
COULSON, A ;
KOZONO, Y ;
LUTTERBACH, B ;
SHOWNKEEN, R ;
SULSTON, J ;
WATERSTON, R .
BIOESSAYS, 1991, 13 (08) :413-417
[7]   MOLECULAR-GENETICS OF KINESIN LIGHT-CHAINS - GENERATION OF ISOFORMS BY ALTERNATIVE SPLICING [J].
CYR, JL ;
PFISTER, KK ;
BLOOM, GS ;
SLAUGHTER, CA ;
BRADY, ST .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (22) :10114-10118
[8]   EVIDENCE THAT THE STALK OF DROSOPHILA KINESIN HEAVY-CHAIN IS AN ALPHA-HELICAL COILED COIL [J].
DECUEVAS, M ;
TAO, T ;
GOLDSTEIN, LSB .
JOURNAL OF CELL BIOLOGY, 1992, 116 (04) :957-965
[9]   NOVEL CYSTEINE-RICH MOTIF AND HOMEODOMAIN IN THE PRODUCT OF THE CAENORHABDITIS-ELEGANS CELL LINEAGE GENE LIN-II [J].
FREYD, G ;
KIM, SK ;
HORVITZ, HR .
NATURE, 1990, 344 (6269) :876-879
[10]   ANALYSIS OF ACCURACY AND IMPLICATIONS OF SIMPLE METHODS FOR PREDICTING SECONDARY STRUCTURE OF GLOBULAR PROTEINS [J].
GARNIER, J ;
OSGUTHORPE, DJ ;
ROBSON, B .
JOURNAL OF MOLECULAR BIOLOGY, 1978, 120 (01) :97-120
1234