NUCLEOTIDE AND DERIVED AMINO-ACID-SEQUENCE OF THE CYANOGENIC BETA-GLUCOSIDASE (LINAMARASE) FROM WHITE CLOVER (TRIFOLIUM-REPENS L)

被引：57

作者：

OXTOBY, E ^{[1
]}

DUNN, MA ^{[1
]}

PANCORO, A ^{[1
]}

HUGHES, MA ^{[1
]}

机构：

[1] UNIV NEWCASTLE UPON TYNE,DEPT BIOCHEM & GENET,NEWCASTLE TYNE NE2 4HH,ENGLAND

来源：

PLANT MOLECULAR BIOLOGY | 1991年 / 17卷 / 02期

关键词：

BETA-GLUCOSIDASE; CYANOGENESIS; LINAMARASE; TRIFOLIUM-REPENS; WHITE CLOVER; DNA SEQUENCE;

D O I：

10.1007/BF00039495

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nucleotide sequence and derived amino acid sequence of two different beta-glucosidase cDNA clones were determined. One clone (TRE104) was identified as the cyanogenic beta-glucosidase by homology with the N-terminal and internal peptide amino acid sequence of the purified enzyme. The biological function of the other beta-glycosidase (TRE361) is not known. Co-segregation of genomic restriction fragments uniquely identified by each cDNA clone shows that these two genes are linked in the white clover genome. Both TRE104 and TRE361 fragments co-segregate with cyanogenic beta-glucosidase activity. Extensive homology was found between the white clover beta-glucosidase sequences and a group of prokaryote and mammalian beta-glycosidases. This group of sequences has no homology with a separate set of beta-glucosidase genes isolated from fungi and the thermophilic bacterium Clostridium thermocellum.

引用

页码：209 / 219

页数：11

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