PURIFICATION OF THE BORRELIA-BURGDORFERI FLAGELLUM BY USE OF A MONOCLONAL-ANTIBODY

被引：5
作者：
FUCHS, R
WILSKE, B
PREACMURSIC, V
SCHIERZ, G
机构：
[1] Max von Pettenkofer Institute, University of Munich, D-8000 Munich 2
来源：
MOLECULAR AND CELLULAR PROBES | 1990年 / 4卷 / 06期
关键词：
affinity chromatography; antigen-specific ELISA; Borrelia burgdorferi; flagella antigen; monoclonal antibody;
D O I：
10.1016/0890-8508(90)90004-J
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The flagellum associated polypeptide p41 is an immunodominant antigen of Borrelia burgdorferi in the early and late stages of Lyme borreliosis. p41 was prepared by affinity chromatography using monoclonal antibody specific for p41. An immunoglobulin class specific ELISA (IgM-, IgG-ELISA) was established with purified p41 as antigen and compared to the conventional ELISA with whole cell ultrasonic antigen. Whereas the sensitivity of IgM- and IgG-ELISA was comparable in both antigen preparations, cross-reactivity of sera from syphilitic patients was reduced in the p41 IgG-ELISA. Discrepant results obtained by use of ultrasonic antigen or p41 antigen, were controlled by Western blots. A correlation between the results of p41-ELISA and Western blot was shown. © 1990.
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页码：451 / 462
页数：12
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