ANALYSIS OF MUTATIONS AT POSITION-184 IN REVERSE-TRANSCRIPTASE OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1

被引：97

作者：

BOYER, PL ^{[1
]}

HUGHES, SH ^{[1
]}

机构：

[1] NCI,FREDERICK CANC RES & DEV CTR,BASIC RES PROGRAM,ADV BIOSCI LABS,FREDERICK,MD 21702

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1995年 / 39卷 / 07期

关键词：

D O I：

10.1128/AAC.39.7.1624

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have analyzed recombinant human immunodeficiency virus type I reverse transcriptases that contain mutations at position 184. These variants retain high levels of RNA-dependent DNA polymerase activity and show resistance to ddITP. However, the mutants varied in their ability to polymerize processively. The variants Met184Ile and Met184Val showed slight reductions in processivity relative to that of the wild-type enzyme; the variants Metl84Ala and Met184Leu showed considerable reductions in their processivity.

引用

页码：1624 / 1628

页数：5

共 42 条

[11] MUTATIONAL SENSITIVITY PATTERNS DEFINE CRITICAL RESIDUES IN THE PALM SUBDOMAIN OF THE REVERSE-TRANSCRIPTASE OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 [J].

CHAO, SF ;

CHAN, VL ;

JURANKA, P ;

KAPLAN, AH ;

SWANSTROM, R ;

HUTCHISON, CA .

NUCLEIC ACIDS RESEARCH, 1995, 23 (05) :803-810

[12] HIV-1 REVERSE-TRANSCRIPTASE PURIFIED FROM A RECOMBINANT STRAIN OF ESCHERICHIA-COLI [J].

CLARK, PK ;

FERRIS, AL ;

MILLER, DA ;

HIZI, A ;

KIM, KW ;

DERINGERBOYER, SM ;

MELLINI, ML ;

CLARK, AD ;

ARNOLD, GF ;

LEBHERZ, WB ;

ARNOLD, E ;

MUSCHIK, GM ;

HUGHES, SH .

AIDS RESEARCH AND HUMAN RETROVIRUSES, 1990, 6 (06) :753-764

[13] HIV RESISTANCE TO REVERSE-TRANSCRIPTASE INHIBITORS [J].

DECLERCQ, E .

BIOCHEMICAL PHARMACOLOGY, 1994, 47 (02) :155-169

[14] ORIGINS AND EVOLUTIONARY RELATIONSHIPS OF RETROVIRUSES [J].

DOOLITTLE, RF ;

FENG, DF ;

JOHNSON, MS ;

MCCLURE, MA .

QUARTERLY REVIEW OF BIOLOGY, 1989, 64 (01) :1-30

[15] THE SAME MUTATION THAT ENCODES LOW-LEVEL HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 RESISTANCE TO 2',3'-DIDEOXYINOSINE AND 2',3'-DIDEOXYCYTIDINE CONFERS HIGH-LEVEL RESISTANCE TO THE (-) ENANTIOMER OF 2',3'-DIDEOXY-3'-THIACYTIDINE [J].

GAO, Q ;

GU, ZX ;

PARNIAK, MA ;

CAMERON, J ;

CAMMACK, N ;

BOUCHER, C ;

WAINBERG, MA .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1993, 37 (06) :1390-1392

[16] NOVEL MUTATION IN THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REVERSE-TRANSCRIPTASE GENE THAT ENCODES CROSS-RESISTANCE TO 2',3'-DIDEOXYINOSINE AND 2',3'-DIDEOXYCYTIDINE [J].

GU, ZX ;

QING, G ;

LI, XG ;

PARNIAK, MA ;

WAINBERG, MA .

JOURNAL OF VIROLOGY, 1992, 66 (12) :7128-7135

[17] IDENTIFICATION AND CHARACTERIZATION OF HIV-SPECIFIC RNASE-H BY MONOCLONAL-ANTIBODY [J].

HANSEN, J ;

SCHULZE, T ;

MELLERT, W ;

MOELLING, K .

EMBO JOURNAL, 1988, 7 (01) :239-243

[18] EXPRESSION OF SOLUBLE, ENZYMATICALLY ACTIVE, HUMAN IMMUNODEFICIENCY VIRUS REVERSE-TRANSCRIPTASE IN ESCHERICHIA-COLI AND ANALYSIS OF MUTANTS [J].

HIZI, A ;

MCGILL, C ;

HUGHES, SH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (04) :1218-1222

[19]

HOWARD KJ, 1991, J BIOL CHEM, V266, P23003

[20]

HUGHES SH, 1990, USE OF X-RAY CRYSTALLOGRAPHY IN THE DESIGN OF ANTIVIRAL AGENTS, P297

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