METHOD FOR DETERMINATION OF PARTITION-COEFFICIENTS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - APPLICATION TO O-HYDROXYLBENZENESULFONANILIDES

The capacity factors (log k') for 26 compounds of widely varying structural types were determined by high-performance liquid chromatography method using methanol-water as mobile phase and ODS column as stationary phase in which free silanol groups were fully suppressed. These determined log k' values are correlated with known partition coefficient (log P-OCT) values of 26 compounds and the regression parameters show that accordingly there are two sets of correlation lines when 26 compounds are divided into two data sets depending on their hydrogen-bonding ability. The factors which caused different partitioning mechanisms of the compounds are discussed. As an application, the partition coefficients of a series of o-hydroxylbenzenesulfonanilides (HBSA) were determined by using obtained relative correlation line and then correlated with their anthelmintic activities.