SEROTYPING OF HUMAN ROTAVIRUSES IN THE TOKYO AREA (1990-1993) BY ENZYME-IMMUNOASSAY WITH MONOCLONAL-ANTIBODIES AND BY REVERSE TRANSCRIPTION AND POLYMERASE CHAIN-REACTION AMPLIFICATION

被引：21

作者：

USHIJIMA, H ^{[1
]}

MUKOYAMA, A ^{[1
]}

HASEGAWA, A ^{[1
]}

NISHIMURA, S ^{[1
]}

KONISHI, K ^{[1
]}

BOSU, K ^{[1
]}

机构：

[1] AKABANE PEDIAT CLIN,TOKYO,JAPAN

来源：

JOURNAL OF MEDICAL VIROLOGY | 1994年 / 44卷 / 02期

关键词：

VP4 AND VP7 GENES; INTRASEROTYPIC VARIATIONS; VACCINE DEVELOPMENT;

D O I：

10.1002/jmv.1890440208

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Serotyping of human rotavirus in the Tokyo area was conducted from 1990 to 1993 by enzyme immunoassay with monoclonal antibodies (EIA-MAbs) against VP7 and by reverse transcription and polymerase chain reaction (RT-PCR) amplification of the VP4 and VP7 genes. The results by EIA-MAbs were very similar to those obtained by RT-PCR. Evidence of intraserotypic variations was suggested because strains of undetermined serotypes were detected by either EIA-MAb or RT-PCR. This kind of study is required for vaccine development. (C) 1994 Wiley-Liss, Inc.

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页码：162 / 165

页数：4

相关论文

共 20 条

[1]

AKATANI K, 1987, CLIN VIROL, V156, P61

[2] ROTAVIRUS DIARRHEA IN BANGLADESHI CHILDREN - CORRELATION OF DISEASE SEVERITY WITH SEROTYPES [J].

BERN, C ;

UNICOMB, L ;

GENTSCH, JR ;

BANUL, N ;

YUNUS, M ;

SACK, RB ;

GLASS, RI .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (12) :3234-3238

[3] A NOVEL GROUP-A ROTAVIRUS-G SEROTYPE - SEROLOGICAL AND GENOMIC CHARACTERIZATION OF EQUINE ISOLATE FI23 [J].

BROWNING, GF ;

FITZGERALD, TA ;

CHALMERS, RM ;

SNODGRASS, DR .

JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (09) :2043-2046

[4]

COULSON BS, 1993, J CLIN MICROBIOL, V31, P1

[5] ROTAVIRUS GENE STRUCTURE AND FUNCTION [J].

ESTES, MK ;

COHEN, J .

MICROBIOLOGICAL REVIEWS, 1989, 53 (04) :410-449

[6] IDENTIFICATION OF GROUP-A ROTAVIRUS GENE-4 TYPES BY POLYMERASE CHAIN-REACTION [J].

GENTSCH, JR ;

GLASS, RI ;

WOODS, P ;

GOUVEA, V ;

GORZIGLIA, M ;

FLORES, J ;

DAS, BK ;

BHAN, MK .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (06) :1365-1373

[7] ISOLATION AND CHARACTERIZATION OF 2 DISTINCT HUMAN ROTAVIRUS STRAINS WITH G6 SPECIFICITY [J].

GERNA, G ;

SARASINI, A ;

PAREA, M ;

ARISTA, S ;

MIRANDA, P ;

BRUSSOW, H ;

HOSHINO, Y ;

FLORES, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (01) :9-16

[8] SEQUENCE OF THE 4TH GENE OF HUMAN ROTAVIRUSES RECOVERED FROM ASYMPTOMATIC OR SYMPTOMATIC INFECTIONS [J].

GORZIGLIA, M ;

GREEN, K ;

NISHIKAWA, K ;

TANIGUCHI, K ;

JONES, R ;

KAPIKIAN, AZ ;

CHANOCK, RM .

JOURNAL OF VIROLOGY, 1988, 62 (08) :2978-2984

[9] ANTIGENIC RELATIONSHIPS AMONG HUMAN ROTAVIRUSES AS DETERMINED BY OUTER CAPSID PROTEIN-VP4 [J].

GORZIGLIA, M ;

LARRALDE, G ;

KAPIKIAN, AZ ;

CHANOCK, RM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (18) :7155-7159

[10] POLYMERASE CHAIN-REACTION AMPLIFICATION AND TYPING OF ROTAVIRUS NUCLEIC-ACID FROM STOOL SPECIMENS [J].

GOUVEA, V ;

GLASS, RI ;

WOODS, P ;

TANIGUCHI, K ;

CLARK, HF ;

FORRESTER, B ;

FANG, ZY .

JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (02) :276-282