A NEW ULTRASENSITIVE BIOLUMINOGENIC ENZYME SUBSTRATE FOR BETA-GALACTOSIDASE

被引：36

作者：

GEIGER, R

SCHNEIDER, E

WALLENFELS, K

MISKA, W

机构：

[1] UNIV FREIBURG,INST ORGAN CHEM & BIOCHEM,W-7800 FREIBURG,GERMANY

[2] UNIV GIESSEN,DERMATOL & ANDROL ABT,W-6300 GIESSEN,GERMANY

来源：

BIOLOGICAL CHEMISTRY HOPPE-SEYLER | 1992年 / 373卷 / 12期

关键词：

D-LUCIFERIN; D-LUCIFERIN DERIVATIVES; D-LUCIFERIN-O-BETA-GALACTOSIDE; LUCIFERASE; BIOLUMINESCENCE; FIREFLY; PHOTINUS-PYRALIS AND BETA-GALACTOSIDASE;

D O I：

10.1515/bchm3.1992.373.2.1187

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A derivative of D-luciferin, D-luciferin-O-beta-galactoside, was synthesized and used as highly sensitive substrate for beta-galactosidase. The substrate was physicochemically characterized. Enzymatic cleavage of the new compound by beta-galactosidase was demonstrated and kinetic constants K(m), V(max), k(cat) and k(cat)/K(m) K(m) have been determined. The compound has been proved to be a highly sensitive substrate for beta-galactosidase, permitting a limit of detection of 3.7 x 10(-19) mol of enzyme per assay.

引用

页码：1187 / 1191

页数：5

共 15 条

[1] USE OF ENZYMES IN IMMUNOASSAY TECHNIQUES - A REVIEW [J].