IMMOBILIZATION OF PENICILLIN-G ACYLASE ON FUNCTIONALIZED MACROPOROUS POLYMER BEADS

被引：33

作者：

BAHULEKAR, RV

PRABHUNE, AA

SIVARAMAN, H

PONRATHNAM, S

机构：

[1] NATL CHEM LAB,DIV CHEM ENGN,POONA 411008,MAHARASHTRA,INDIA

[2] NATL CHEM LAB,DIV BIOCHEM SCI,POONA 411008,MAHARASHTRA,INDIA

来源：

POLYMER | 1993年 / 34卷 / 01期

关键词：

BIOCATALYST ENZYME IMMOBILIZATION; MACROPOROUS SUPPORT; POLYETHYLENEIMINE; PENICILLIN ACYLASE;

D O I：

10.1016/0032-3861(93)90300-Y

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Macroporous beaded polymers of varying pore sizes were synthesized coated with polyethyleneimine and derivatized with glutaraldehyde to generate aldehyde pendant groups. Penicillin G acylase (EC 3.5.1.11) was immobilized by covalent binding and the performance of immobilized enzyme was evaluated in batch mode. The expression was estimated as 43%. The shift in pH optima was marginal. The optimum temperature shifted from 40 to 57-degrees-C and K(m) shifted from 31 to 12.8 mumol. Immobilization enhanced the thermal stability of the enzyme.

引用

页码：163 / 166

页数：4

共 8 条

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