BEHAVIOR OF POLYHYDROXYETHYL METHACRYLATE SORBENT WITH DEXTRAN-FILLED MACROPORES IN DYE-AFFINITY CHROMATOGRAPHY OF PROTEINS

被引：8
作者：
MISLOVICOVA, D
PETRO, M
BEREK, D
机构：
[1] COMENIUS UNIV BRATISLAVA, FAC SCI, DEPT ANALYT CHEM, CS-84215 BRATISLAVA, CZECHOSLOVAKIA
[2] SLOVAK ACAD SCI, INST POLYMER, CS-84238 BRATISLAVA, CZECHOSLOVAKIA
来源：
JOURNAL OF CHROMATOGRAPHY | 1993年 / 646卷 / 02期
关键词：
D O I：
10.1016/0021-9673(93)83355-V
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A series of composite sorbents based on the rigid macroporous polyhydroxyethyl methacrylate (HEMA)-based support filled with soft dextran gel was prepared. The affinity dyes Cibacron Blue and Remazol Brilliant Blue were then immobilized on both the original HEMA and the composite (HEMA-D) sorbents. The properties of HEMA and HEMA-D sorbents, such as hydrophobic interactions with proteins and recovery of chromatographed enzymes (lactate dehydrogenase and malate dehydrogenase), in both original and dyed forms were studied. Important advantages of using HEMA-D supports in dye-affinity chromatography as compared with only HEMA supports were found.
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页码：411 / 416
页数：6
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