CHARACTERIZATION OF BACTERIA BY MULTIPARAMETER FLOW-CYTOMETRY

被引：48

作者：

ALLMAN, R

HANN, AC

MANCHEE, R

LLOYD, D

机构：

[1] UNIV WALES COLL CARDIFF,DEPT PHYSIOL,PRECLIN ELECTRON MICROSCOPY UNIT,CARDIFF CF1 1XL,S GLAM,WALES

[2] CHEM DEF ESTAB,SALISBURY SP4 0JQ,WILTS,ENGLAND

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1992年 / 73卷 / 05期

关键词：

D O I：

10.1111/j.1365-2672.1992.tb05001.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An arc-lamp based flow cytometer was used to obtain high resolution measurements of the light scattering characteristics and DNA contents of eight different bacteria. Light scatter profiles of bacteria are a useful first step when flow cytometry is used to characterize organisms. Scanning and transmission electron microscopy of the bacterial samples demonstrate that the structural basis of the light scattering profiles is not always clear, i.e. some organisms appear to have anomalous light scattering characteristics. The use of a third measurement parameter, DNA content, allowed much better discrimination of the organisms. Flow cytometry shows great promise as a method for the rapid discrimination and identification of bacterial populations.

引用

页码：438 / 444

页数：7

共 14 条

[1] GROWTH OF AZOTOBACTER-VINELANDII WITH CORRELATION OF COULTER CELL-SIZE, FLOW CYTOMETRIC PARAMETERS, AND ULTRASTRUCTURE [J].