In planta Agrobacterium-MEDIATED GENETIC TRANSFORMATION IN OKRA {Abelmoschus esculentus (L.) Moench}

被引：4
|
作者：
Menon, Rohit [1 ]
Sarao, Navraj Kaur [1 ]
Pathak, Mamta [2 ]
机构：
[1] Punjab Agr Univ, Sch Agr Biotechnol, Ludhiana 141004, Punjab, India
[2] Punjab Agr Univ, Dept Vegetable Sci, Ludhiana 141004, Punjab, India
来源：
APPLIED BIOLOGICAL RESEARCH | 2018年 / 20卷 / 03期
关键词：
Abelmoschus esculentus; Bacillus thuringiensis; shoot and fruit borer; transgenic okra;
D O I：
10.5958/0974-4517.2018.00030.7
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
In Planta methods have been developed in several plant species to overcome the problems associated with their tissue culture-based transformation. In present study, a method for genetic transformation in okra (Abelmoschus esculentus) was established using in planta Agrobacterium-mediated genetic transformation. The embryos of imbibed seeds pricked from meristematic region of plumule were used as explants for transformation. Agrobacterium tumefaciens strain EHA105 was carrying cry1Ac gene against fruit and shoot borer, CaMV 35S as promoter and nptII as plant selectable marker gene. The putative transgenic plants were confirmed by amplifying transgene through polymerase chain reaction.
引用
收藏
页码：221 / 227
页数：7
相关论文
共 50 条
  • [31] Effects of soil amendments on the nutritional quality of okra (Abelmoschus esculentus [L.] Moench)
    Adewole, M. B.
    Ilesanmi, A. O.
    JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION, 2011, 11 (03) : 45 - 55
  • [32] TETRAZOLIUM TEST TO ESTIMATE OKRA (Abelmoschus esculentus (L.) MOENCH) SEED VIABILITY
    Torres Souza, Antonia Adailha
    Nogueira, Narjara Walessa
    Torres, Salvador Barros
    Benedito, Clarisse Pereira
    de Souza Neta, Maria Lilia
    de Paiva, Emanoela Pereira
    REVISTA CAATINGA, 2018, 31 (04) : 817 - 822
  • [33] Impact of nitrogen and spacing on the growth and yield of okra [Abelmoschus esculentus (L.) Moench]
    Brar, Navdeep Singh
    Singh, Daljeet
    4TH INTERNATIONAL CONFERENCE ON ADVANCEMENTS IN ENGINEERING & TECHNOLOGY (ICAET-2016), 2016, 57
  • [34] ESTIMATION OF LEAF IN OKRA (ABELMOSCHUS-ESCULENTUS L) MOENCH
    ASIF, MI
    TROPICAL AGRICULTURE, 1977, 54 (02): : 192 - 192
  • [35] Effect of micronutrient supplement in growth and development of okra (Abelmoschus esculentus, (L.) Moench)
    Rafique, AKMA
    Muhsi, AAA
    BANGLADESH JOURNAL OF BOTANY, 2004, 33 (02): : 129 - 131
  • [36] INFLUENCE OF RHIZOBACTERIA AND ORGANIC MANURES ON YIELD OF OKRA ( ABELMOSCHUS ESCULENTUS (L.) MOENCH)
    Dhiman, Shivali
    Kumari, Santosh
    Dogra, Balbir singh
    Sohi, Anuj
    Chaudhary, Parveen
    Saini, Anubha
    BANGLADESH JOURNAL OF BOTANY, 2024, 53 (04): : 1021 - 1025
  • [37] Development of New High-yielding Okra (Abelmoschus esculentus L. Moench)
    Adetula, Olagorite
    HORTSCIENCE, 2019, 54 (09) : S124 - S124
  • [38] Characterisation of cell wall polysaccharides from okra (Abelmoschus esculentus (L.) Moench)
    Sengkhamparn, Nipaporn
    Verhoef, Rene
    Schols, Henk A.
    Sajjaanantakul, Tanaboon
    Voragen, Alphons G. J.
    CARBOHYDRATE RESEARCH, 2009, 344 (14) : 1824 - 1832
  • [39] STUDIES ON HETEROSIS FOR EARLINESS IN SPRING SEASON OKRA (Abelmoschus esculentus L. Moench)
    Jindal, S. K.
    Arora, Deepak
    Ghai, T. R.
    SABRAO JOURNAL OF BREEDING AND GENETICS, 2010, 42 (02): : 65 - 73
  • [40] Performance and Genetic Variability of Okra (Abelmoschus esculentus (L.) Moench) Genotypes in Ethiopia for Agromorphology and Biochemical Traits
    Mohammed, Jemal
    Mohammed, Wassu
    Shiferaw, Eleni
    ADVANCES IN AGRICULTURE, 2022, 2022
12345