In planta Agrobacterium-MEDIATED GENETIC TRANSFORMATION IN OKRA {Abelmoschus esculentus (L.) Moench}

被引：4
|
作者：
Menon, Rohit [1 ]
Sarao, Navraj Kaur [1 ]
Pathak, Mamta [2 ]
机构：
[1] Punjab Agr Univ, Sch Agr Biotechnol, Ludhiana 141004, Punjab, India
[2] Punjab Agr Univ, Dept Vegetable Sci, Ludhiana 141004, Punjab, India
来源：
APPLIED BIOLOGICAL RESEARCH | 2018年 / 20卷 / 03期
关键词：
Abelmoschus esculentus; Bacillus thuringiensis; shoot and fruit borer; transgenic okra;
D O I：
10.5958/0974-4517.2018.00030.7
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
In Planta methods have been developed in several plant species to overcome the problems associated with their tissue culture-based transformation. In present study, a method for genetic transformation in okra (Abelmoschus esculentus) was established using in planta Agrobacterium-mediated genetic transformation. The embryos of imbibed seeds pricked from meristematic region of plumule were used as explants for transformation. Agrobacterium tumefaciens strain EHA105 was carrying cry1Ac gene against fruit and shoot borer, CaMV 35S as promoter and nptII as plant selectable marker gene. The putative transgenic plants were confirmed by amplifying transgene through polymerase chain reaction.
引用
收藏
页码：221 / 227
页数：7
相关论文
共 50 条
  • [1] Assessment of factors influencing the tissue culture-independent Agrobacterium-mediated in planta genetic transformation of okra [Abelmoschus esculentus (L.) Moench]
    Markandan Manickavasagam
    Kondeti Subramanyam
    Rajagobalan Ishwarya
    Dhandapani Elayaraja
    Andy Ganapathi
    Plant Cell, Tissue and Organ Culture (PCTOC), 2015, 123 : 309 - 320
  • [2] Assessment of factors influencing the tissue culture-independent Agrobacterium-mediated in planta genetic transformation of okra [Abelmoschus esculentus (L.) Moench]
    Manickavasagam, Markandan
    Subramanyam, Kondeti
    Ishwarya, Rajagobalan
    Elayaraja, Dhandapani
    Ganapathi, Andy
    PLANT CELL TISSUE AND ORGAN CULTURE, 2015, 123 (02) : 309 - 320
  • [3] Production of Transgenic Okra (Abelmoschus esculentus L.) by Agrobacterium-mediated Transformation and Inheritance of Transgene
    Narendran, M.
    Deole, Satish G.
    Char, Bharat R.
    Zehr, Usha B.
    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 2013, 49 (04) : 479 - 479
  • [4] Effective Agrobacterium-mediated genetic transformation of okra (Abelmoschus esculentus L.) and generation of RNAi plants resistant to Begomovirus infecting okra
    Sharma, Nity
    Sarao, Navraj Kaur
    Mohanpuria, Prashant
    Sharma, Abhishek
    JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY, 2024, 99 (02): : 146 - 159
  • [5] Genetic variability and heritability in cultivated okra [Abelmoschus esculentus (L.) Moench]
    Nwangburuka, C. C.
    Denton, O. A.
    Kehinde, O. B.
    Ojo, D. K.
    Popoola, A. R.
    SPANISH JOURNAL OF AGRICULTURAL RESEARCH, 2012, 10 (01) : 123 - 129
  • [6] Genetic control of aluminium tolerance in okra (Abelmoschus esculentus (L.) Moench)
    Singh, D.
    SCIENTIA HORTICULTURAE, 2012, 138 : 134 - 137
  • [7] Stability analysis in okra [Abelmoschus esculentus (L.) Moench]
    Dabhi, K. H.
    Vachhani, J. H.
    Poshiya, V. K.
    Jivani, L. L.
    Chitaroda, J. D.
    RESEARCH ON CROPS, 2010, 11 (02) : 391 - 396
  • [8] Studies on the genetic causes of heterosis in Okra [Abelmoschus esculentus (L.) Moench.]
    Eswaran, R.
    Thirugnanakumar, S.
    Sampathkumar, C. Praveen
    Anandan, A.
    Padmanaban, C.
    PLANT ARCHIVES, 2007, 7 (02): : 721 - 724
  • [9] Estimation of heritability and genetic advance in Okra [Abelmoschus esculentus (L.) Moench.]
    Kumar, Sunil
    Yadav, J. R.
    Mishra, Gaurav
    Kumar, Sanjeev
    Singh, B.
    PLANT ARCHIVES, 2007, 7 (02): : 923 - 924
  • [10] Correlation coefficient analysis in Okra [Abelmoschus Esculentus (L.) Moench]
    Yadav, J. R.
    Singh, B.
    Mishra, Gaurav
    Kumar, Sanjeev
    Kumar, Sunil
    PLANT ARCHIVES, 2007, 7 (01): : 307 - 308
12345