CHEMICAL SYNTHESIS AND QUANTITATIVE ESTIMATION OF 3-DEOXY D-MANNO-OCTULOSONIC ACID

被引：31

作者：

CHARON, D

SARFATI, RS

STROBACH, DR

SZABO, L

机构：

[1] Institut de Biochimie, Faculté des Sciences, Orsay

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1969年 / 11卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1969.tb00781.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

3‐Deoxy d‐manno‐octulosonic acid has been synthesised starting from d‐mannose via 2‐deoxy d‐manno heptose, cyanohydrine addition, and selective oxidation of the epimeric hydroxyl group of the resulting 3‐deoxy octonic acids. When treated with periodate in 0.1 N sulphuric acid solution at 0°, the deoxy octulosonic acid and other 3‐deoxy aldulosonic acids all yield one molar equivalent of β‐formyl pyruvate. Conditions are described in which β‐formyl pyruvate can be estimated by the thiobarbituric acid method; hence, when treated successively with periodate and thiobarbiturate, all 3‐deoxy aldulosonic acids have the same molar extinction coefficient [(92 ± 5)103 in dilute acid; 133 × 103 in cyclohexanone] and can be estimated by this method without the use of standards. Copyright © 1969, Wiley Blackwell. All rights reserved

引用

页码：364 / &

共 42 条

[1] METHODS FOR QUANTITATIVE ESTIMATION OF N-ACETYLNEURAMINIC ACID AND THEIR APPLICATION TO HYDROLYSATES OF SIALOMUCOIDS [J].