QUANTITATIVE DETECTION OF HIV-1 DRUG-RESISTANCE MUTATIONS BY AUTOMATED DNA-SEQUENCING

被引：173
作者：
LARDER, BA
KOHLI, A
KELLAM, P
KEMP, SD
KRONICK, M
HENFREY, RD
机构：
[1] APPL BIOSYST 850,FOSTER CITY,CA 94404
[2] APPL BIOSYST LTD,WARRINGTON WA3 7PB,CHESHIRE,ENGLAND
来源：
NATURE | 1993年 / 365卷 / 6447期
关键词：
D O I：
10.1038/365671a0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A comparison has been made between manual and automated DNA sequencing procedures to evaluate the ability to distinguish mixtures of wild-type and mutant sequences. Quantitative detection of such mixtures of HIV-1 drug resistance mutations was best achieved using an automated system that uses fluorescent-labelled sequencing primers. This procedure has a wide range of applications in clinical research, including heterozygote analysis. Software that automatically reports mixed-base positions is presented.
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页码：671 / 673
页数：3
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