In this paper, Langmuir-Blodgett films of hematoporphyrin mixed with stearic acid have been investigated. It was found that hematoporphyrin molecules were inlaid in monolayers of stearic acid. The UV-vis spectra of the LB films showed that there wasn't strong interaction between hematoporphyrin molecules when compared with the solution spectrum. The particular orientation of hematoporphyrin macrocycle in LB film was confirmed by polarized UV-vis spectra.