NEISSERIA-MENINGITIDIS TRANSFERRIN-BINDING PROTEIN-1 EXPRESSED IN ESCHERICHIA-COLI IS SURFACE-EXPOSED AND BINDS HUMAN TRANSFERRIN

被引：18
作者：
PALMER, HM
POWELL, NBL
ALAALDEEN, DA
WILTON, J
BORRIELLO, SP
机构：
来源：
FEMS MICROBIOLOGY LETTERS | 1993年 / 110卷 / 02期
关键词：
NEISSERIA-MENINGITIDIS; TRANSFERRIN; POLYMERASE CHAIN REACTION; TRANSFERRIN BINDING PROTEIN;
D O I：
10.1016/0378-1097(93)90456-C
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A gene library of Neisseria meningitidis B15 P1.16 DNA was established in lambdaZap II and clones containing DNA encoding transferrin binding protein 1 (TBP-1) identified following hybridisation with a 63-bp DNA probe based on the codon assignment for the first 21 N-terminal amino acids of TBP-1. Sequencing of the cloned DNA demonstrated that all of the intergenic DNA (i.e. upstream of tbp-1 running through to the 3' end of the transferrin-binding protein 2 gene) and approx. 15% of tbp-1 had been cloned. The complete gene was generated using a polymerase chain reaction, with the primer for the 3' end being based on tbp-A of N. gonorrhoeae, and the approx. 2.9-kb DNA product cloned into pGem-3Z. The expressed protein (approx. 100 kDa) reacted with antiserum to an N-terminal peptide of TBP-1. In addition, the native product was surface-expressed by Escherichia coli and bound human transferrin.
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页码：139 / 146
页数：8
相关论文
共 19 条
[1]   THE 70 KILODALTON IRON REGULATED PROTEIN OF NEISSERIA-MENINGITIDIS IS NOT THE HUMAN TRANSFERRIN RECEPTOR [J].
ALAALDEEN, DA ;
DAVIES, HA ;
WALL, RA ;
BORRIELLO, SP .
FEMS MICROBIOLOGY LETTERS, 1990, 69 (1-2) :37-42
[2]  
ALAALDEEN DA, 1991, NEISSERIAE 1990, P415
[3]   LOCALIZATION OF THE MENINGOCOCCAL RECEPTORS FOR HUMAN TRANSFERRIN [J].
ALAALDEEN, DAA ;
POWELL, NBL ;
WALL, RA ;
BORRIELLO, SP .
INFECTION AND IMMUNITY, 1993, 61 (02) :751-759
[4]   GENETIC-EVIDENCE THAT NEISSERIA-GONORRHOEAE PRODUCES SPECIFIC RECEPTORS FOR TRANSFERRIN AND LACTOFERRIN [J].
BLANTON, KJ ;
BISWAS, GD ;
TSAI, J ;
ADAMS, J ;
DYER, DW ;
DAVIS, SM ;
KOCH, GG ;
SEN, PK ;
SPARLING, PF .
JOURNAL OF BACTERIOLOGY, 1990, 172 (09) :5225-5235
[5]   ANALYSIS OF THE CLONAL RELATIONSHIPS BETWEEN STRAINS OF NEISSERIA-MENINGITIDIS BY PULSED FIELD GEL-ELECTROPHORESIS [J].
BYGRAVES, JA ;
MAIDEN, MCJ .
JOURNAL OF GENERAL MICROBIOLOGY, 1992, 138 :523-531
[6]   GONOCOCCAL TRANSFERRIN-BINDING PROTEIN-1 IS REQUIRED FOR TRANSFERRIN UTILIZATION AND IS HOMOLOGOUS TO TONB-DEPENDENT OUTER-MEMBRANE RECEPTORS [J].
CORNELISSEN, CN ;
BISWAS, GD ;
TSAI, J ;
PARUCHURI, DK ;
THOMPSON, SA ;
SPARLING, PF .
JOURNAL OF BACTERIOLOGY, 1992, 174 (18) :5788-5797
[7]   CHARACTERIZATION OF 2 GENES ENCODING DISTINCT TRANSFERRIN-BINDING PROTEINS IN DIFFERENT ACTINOBACILLUS-PLEUROPNEUMONIAE ISOLATES [J].
GERLACH, GF ;
KLASHINSKY, S ;
ANDERSON, C ;
POTTER, AA ;
WILLSON, PJ .
INFECTION AND IMMUNITY, 1992, 60 (08) :3253-3261
[8]   ANTIGENIC RELATIONSHIPS OF TRANSFERRIN-BINDING PROTEINS FROM NEISSERIA-MENINGITIDIS, N-GONORRHEA AND HAEMOPHILUS-INFLUENZAE - CROSS-REACTIVITY OF ANTIBODIES TO NH(2)-TERMINAL PEPTIDES [J].
GRIFFITHS, E ;
STEVENSON, P ;
BYFIELD, P ;
ALAALDEEN, DA ;
BORRIELLO, SP ;
HOLLAND, J ;
PARSONS, T ;
WILLIAMS, P .
FEMS MICROBIOLOGY LETTERS, 1993, 109 (01) :85-92
[9]   ANTIGENIC AND MOLECULAR HETEROGENEITY OF THE TRANSFERRIN-BINDING PROTEIN OF NEISSERIA-MENINGITIDIS [J].
GRIFFITHS, E ;
STEVENSON, P ;
RAY, A .
FEMS MICROBIOLOGY LETTERS, 1990, 69 (1-2) :31-36
[10]   CONSTRUCTION OF T-VECTORS, A RAPID AND GENERAL SYSTEM FOR DIRECT CLONING OF UNMODIFIED PCR PRODUCTS [J].
MARCHUK, D ;
DRUMM, M ;
SAULINO, A ;
COLLINS, FS .
NUCLEIC ACIDS RESEARCH, 1991, 19 (05) :1154-1154
12