MECHANICAL-PROPERTIES OF VESICLES .1. COORDINATED ANALYSES OF OSMOTIC SWELLING AND LYSIS

被引:79
作者
ERTEL, A
MARANGONI, AG
MARSH, J
HALLETT, FR
WOOD, JM
机构
[1] UNIV GUELPH,DEPT MICROBIOL,GUELPH N1G 2W1,ONTARIO,CANADA
[2] UNIV GUELPH,GUELPH WATERLOO CTR GRAD WORK CHEM,GUELPH N1G 2W1,ONTARIO,CANADA
[3] UNIV GUELPH,GUELPH WATERLOO PROGRAM GRAD WORK PHYS,GUELPH N1G 2W1,ONTARIO,CANADA
关键词
D O I
10.1016/S0006-3495(93)81383-3
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
To determine how transmembrane osmotic gradients perturb the structure and dynamics of biological membranes, we examined the effects of medium dilution on the structures of osmolyte-loaded lipid vesicles. Our preparations were characterized by dynamic light scattering (DLS) and nuclear magnetic resonance (NMR) spectroscopies. Populations of Escherichia coli phosphatidyl-ethanolamine (PE) or dioleoylphosphatidylglycerol (DOPG) vesicles prepared by the pH jump technique were variable and polymodal in size distribution. Complex and variable structural changes occurred when PE vesicles were diluted with hypotonic buffer. Such vesicles could not be used as model systems for the analysis of membrane mechanical properties. NaCl-loaded, DOPG vesicles prepared by extrusion through 100 nm (diameter) pores were reproducible and monomodal in size distribution and unilamellar, whereas those prepared by extrusion through 200-, 400-, or 600-nm pores were variable and polymodal in size distribution and/or multilamellar. Time and pressure regimes associated with osmotic lysis of extruded vesicles were defined by monitoring release of carboxyfluorescein, a self-quenching fluorescent dye. Corresponding effects of medium dilution on vesicle structure were assessed by DLS spectroscopy. These experiments and the accompanying analysis (Hallett, F. R., J. Marsh, B. G. Nickel, and J. M. Wood. 1993. Biophys. J. 64:000-000) revealed conditions under which vesicles are expected to reside in a consistently strained state.
引用
收藏
页码:426 / 434
页数:9
相关论文
共 50 条
[41]   STUDIES ON POLYPROPYLENE COMPOSITES FILLED WITH TALC PARTICLES .1. MECHANICAL-PROPERTIES [J].
MAITI, SN ;
SHARMA, KK .
JOURNAL OF MATERIALS SCIENCE, 1992, 27 (17) :4605-4613
[42]   ADAPTION OF SENSORY TEXTURE PROFILE ANALYSIS .1. SCALING OF MECHANICAL-PROPERTIES [J].
ROHM, H ;
VEITS, V .
ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG, 1989, 189 (06) :538-543
[43]   MECHANICAL-PROPERTIES OF GLOBULAR PROTEIN GELS .1. INCIPIENT GELATION BEHAVIOR [J].
RICHARDSON, RK ;
ROSSMURPHY, SB .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 1981, 3 (05) :315-322
[44]   MICROPOROUS MATERIALS WITH NEGATIVE POISSON RATIOS .1. MICROSTRUCTURE AND MECHANICAL-PROPERTIES [J].
CADDOCK, BD ;
EVANS, KE .
JOURNAL OF PHYSICS D-APPLIED PHYSICS, 1989, 22 (12) :1877-1882
[45]   THE EFFECTS OF CHROMIUM ON NIAL INTERMETALLIC ALLOYS .1. MICROSTRUCTURES AND MECHANICAL-PROPERTIES [J].
COTTON, JD ;
NOEBE, RD ;
KAUFMAN, MJ .
INTERMETALLICS, 1993, 1 (01) :3-20
[46]   GLASS BEAD-FILLED POLYPROPYLENE .1. RHEOLOGICAL AND MECHANICAL-PROPERTIES [J].
FAULKNER, DL ;
SCHMIDT, LR .
POLYMER ENGINEERING AND SCIENCE, 1977, 17 (09) :657-665
[47]   A VISCOELASTIC ANALYSIS OF THE KERATIN COMPOSITE .1. LONGITUDINAL AND TRANSVERSE MECHANICAL-PROPERTIES [J].
TAO, XM ;
POSTLE, R .
TEXTILE RESEARCH JOURNAL, 1989, 59 (03) :123-138
[48]   FINELY DISPERSE CEMENTED CARBIDES WC NI .1. MECHANICAL-PROPERTIES [J].
SVERDEL, VV ;
SHATOV, AV ;
YURCHUK, NA ;
BAKUN, OV .
POWDER METALLURGY AND METAL CERAMICS, 1994, 33 (1-2) :68-70
[49]   CARBON, A PROMISING MATERIAL IN ENDOPROSTHETICS .1. THE CARBON MATERIALS AND THEIR MECHANICAL-PROPERTIES [J].
BRUCKMANN, H ;
HUTTINGER, KJ .
BIOMATERIALS, 1980, 1 (02) :67-72
[50]   DEFORMATION CHARACTERISTICS OF REINFORCED EPOXY-RESIN .1. MECHANICAL-PROPERTIES [J].
PINK, E ;
CAMPBELL, JD .
JOURNAL OF MATERIALS SCIENCE, 1974, 9 (04) :658-664