THE LARGE-SCALE ISOLATION OF BRYOSTATIN-1 FROM BUGULA-NERITINA FOLLOWING CURRENT GOOD MANUFACTURING PRACTICES

被引：115

作者：

SCHAUFELBERGER, DE

KOLECK, MP

BEUTLER, JA

VATAKIS, AM

ALVARADO, AB

ANDREWS, P

MARZO, LV

MUSCHIK, GM

ROACH, J

ROSS, JT

LEBHERZ, WB

REEVES, MP

EBERWEIN, RM

RODGERS, LL

TESTERMAN, RP

SNADER, KM

FORENZA, S

机构：

[1] NCI,PRI DYNCORP,FERMENTAT PROD FACIL,CHEM SYNTH & ANAL LAB,FREDERICK CANC RES & DEV CTR,POB B,FREDERICK,MD 21702

[2] NCI,DIV CANC TREATMENT,DTP,NAT PROD BRANCH,FREDERICK CANC RES & DEV CTR,FREDERICK,MD 21702

[3] BRISTOL MYERS SQUIBB CO,PHARMACEUT RES & DEV,WALLINGFORD,CT 06492

来源：

JOURNAL OF NATURAL PRODUCTS | 1991年 / 54卷 / 05期

关键词：

D O I：

10.1021/np50077a004

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A novel process was designed for the large-scale isolation of bryostatin 1 {1} from the bryozoan Bugula neritina L. in order to obtain multigram quantities of highly pure material for formulation studies, preclinical toxicology, and clinical trials in cancer patients. Multigram quantities of bryostatin 1 were obtained from a collection of approximately 10,000 gallons of wet animal. A phorbol dibutyrate (PDBu) receptor binding assay and hplc with photodiode array detection were used for the design, validation, and control of the isolation process.

引用

页码：1265 / 1270

页数：6

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