SIMULTANEOUS MASS-SPECTROMETRIC MEASUREMENT OF PROSTAGLANDINS-E1 (PGE1) AND PGE2 WITH A DEUTERATED INTERNAL STANDARD

被引：11

作者：

FERRETTI, A ^{[1
]}

FLANAGAN, VP ^{[1
]}

机构：

[1] USDA SEA, BELTSVILLE AGR RES CTR, CTR HUMAN NUTR, INST NUTR, LIPID NUTR LAB, BELTSVILLE, MD 20705 USA

来源：

LIPIDS | 1979年 / 14卷 / 05期

关键词：

D O I：

10.1007/BF02533466

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of (3,3,4,4-2H4)PGE2 as internal standard for the quantitative measurement of PGE1 by gas chromatography-mass spectrometry was reported. A method for the simultaneous analysis of PGE1 and PGE2 was also described. The PG were analyzed by selected ion monitoring as the methyl ester-trimethylsilyl ether (ME-TMS) derivatives of PGB1 and PGB2, respectively. A linear response over the range 1-70 ng (2.8-198 pmoles) was demonstrated in all cases. The use of this procedure is exemplified by its application to the PG analysis of sheep seminal vesicles.

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页码：483 / 491

页数：9

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