Synthesis, Characterization, Antioxidant and Antimicrobial Activities and DNA Damage of Some Novel 2-[3-alkyl (aryl)-4,5-dihydro-1H-1,2,4-triazol-5-one-4-yl]-phenoxyacetic acids in Human Lymphocytes

In this study, nine novel 2-[3-alkyl(aryl)-4,5-dihydro-1H-1,2,4-triazol-5-one-4-yl] phenoxyacetic acids (2) were obtained by the reactions of 3-alkyl-4-amino-4,5-dihydro-1H-1,2,4-triazol-5-ones with 2-formylphenoxy acetic acid. Their structures were characterized by using H-1-NMR, C-13-NMR and IR spectral data together with elemental analysis. The synthesized novel compounds were analyzed for their in vitro antioxidant activity methods such as reducing power, free radical scavenging and metal chelating activity. Besides, agar hole methods were used to investigate the antibacterial properties of the 2 type compounds against the strains of B. subtilis, B. cereus, P. aeruginosa, K. Pneumoniae, S. aureus, and E. coli. Finally, the alkaline comet assay was used to investigate genotoxicity potential of the synthesized compounds in the peripheral blood lymphocytes (PBLs).