ADSORPTION BEHAVIOR OF ALBUMIN AND CONALBUMIN ON TSK-DEAE 5PW ANION-EXCHANGER

The adsorption behavior of chicken albumin and conalbumin on TSK-DEAE 5PW ion exchanger was studied by determining the equilibrium isotherms of these compounds at different temperatures, with different mobile phase ionic strengths and pH, using frontal analysis. The last two parameters have a considerable influence on the isotherms, including the saturation capacity, whereas temperature has a much less important influence. In most instances, and especially with chicken albumin, these isotherms are poorly approximated by a Langmuir isotherm equation, but rather correspond to high-affinity isotherm types. The Scatchard plots exhibit a downward convexity, indicating non-cooperative adsorption on two kinds of sites. The adsorption process tends to be partially irreversible towards dilution in the mobile phase. © 1990.