EVIDENCE THAT THE F-2-ISOPROSTANE, 8-EPI-PROSTAGLANDIN F2-ALPHA, IS FORMED IN-VIVO

被引：152

作者：

MORROW, JD

MINTON, TA

BADR, KF

ROBERTS, LJ

机构：

[1] VANDERBILT UNIV,DEPT MED,NASHVILLE,TN 37232

[2] EMORY UNIV,DEPT MED,DIV NEPHROL,ATLANTA,GA 30033

[3] VET AFFAIRS MED CTR,ATLANTA,GA 30033

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1994年 / 1210卷 / 02期

关键词：

ISOPROSTANE; PROSTAGLANDIN; EICOSANOID; LIPID PEROXIDATION; FREE RADICAL;

D O I：

10.1016/0005-2760(94)90128-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

F-2-isoprostanes are prostaglandin (PG)F-2-like compounds that are produced in vivo as non-enzymatic products of free radical catalyzed peroxidation of arachidonic acid. One F-2-isoprostane whose formation should be favored is 8-epi-PGF(2 alpha), 8-Epi-PGF(2 alpha) has been shown to exert potent bioactivity but proof that it is formed in vivo is lacking. Evidence is now presented suggesting that 8-epi-PGF(2 alpha) is, in fact, formed in vivo by demonstrating that an endogenous F-2-isoprostane with a retention time on capillary GC identical with that of 8-epi-PGF(2 alpha) co-chromatographs through four high resolving HPLC purification procedures with authentic radiolabelled 8-epi-PGF(2 alpha).

引用

收藏

页码：244 / 248

页数：5

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