THE SECONDARY STRUCTURE OF BACTERIORHODOPSIN IN ORGANIC SOLUTION - A FOURIER-TRANSFORM INFRARED STUDY

被引：12
|
作者：
TORRES, J [1 ]
PADROS, E [1 ]
机构：
[1] UNIV AUTONOMA BARCELONA,FAC MED,DEPT BIOQUIM & BIOL MOLEC,UNITAT BIOFIS,E-08193 BARCELONA,SPAIN
来源：
FEBS LETTERS | 1993年 / 318卷 / 01期
关键词：
BACTERIORHODOPSIN; SECONDARY STRUCTURE; INFRARED SPECTROSCOPY; ALPHA-HELIX; REVERSE TURN; ORGANIC SOLVENT;
D O I：
10.1016/0014-5793(93)81331-S
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Fourier transform infrared spectroscopy is used to estimate the secondary structure of bacteriorhodopsin dissolved in chloroform-methanol (1:1 v/v), 0.1 M LiClO4. Curve-fitting of the deconvolved spectra in the amide I region shows that the total content of alpha-helices, reverse turns and beta-sheets are similar to the native state. However, the alpha(II)-helices, which are the major helical class in native bacteriorhodopsin, are greatly decreased in the solubilized sample. Similarly, the reverse turns and the beta-sheets are strongly altered.
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页码：77 / 79
页数：3
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