THE SECONDARY STRUCTURE OF BACTERIORHODOPSIN IN ORGANIC SOLUTION - A FOURIER-TRANSFORM INFRARED STUDY

被引：12

作者：

TORRES, J ^{[1
]}

PADROS, E ^{[1
]}

机构：

[1] UNIV AUTONOMA BARCELONA,FAC MED,DEPT BIOQUIM & BIOL MOLEC,UNITAT BIOFIS,E-08193 BARCELONA,SPAIN

来源：

FEBS LETTERS | 1993年 / 318卷 / 01期

关键词：

BACTERIORHODOPSIN; SECONDARY STRUCTURE; INFRARED SPECTROSCOPY; ALPHA-HELIX; REVERSE TURN; ORGANIC SOLVENT;

D O I：

10.1016/0014-5793(93)81331-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fourier transform infrared spectroscopy is used to estimate the secondary structure of bacteriorhodopsin dissolved in chloroform-methanol (1:1 v/v), 0.1 M LiClO4. Curve-fitting of the deconvolved spectra in the amide I region shows that the total content of alpha-helices, reverse turns and beta-sheets are similar to the native state. However, the alpha(II)-helices, which are the major helical class in native bacteriorhodopsin, are greatly decreased in the solubilized sample. Similarly, the reverse turns and the beta-sheets are strongly altered.

引用

页码：77 / 79

页数：3