THE SECONDARY STRUCTURE OF BACTERIORHODOPSIN IN ORGANIC SOLUTION - A FOURIER-TRANSFORM INFRARED STUDY

被引：12

作者：

TORRES, J ^{[1
]}

PADROS, E ^{[1
]}

机构：

[1] UNIV AUTONOMA BARCELONA,FAC MED,DEPT BIOQUIM & BIOL MOLEC,UNITAT BIOFIS,E-08193 BARCELONA,SPAIN

来源：

FEBS LETTERS | 1993年 / 318卷 / 01期

关键词：

BACTERIORHODOPSIN; SECONDARY STRUCTURE; INFRARED SPECTROSCOPY; ALPHA-HELIX; REVERSE TURN; ORGANIC SOLVENT;

D O I：

10.1016/0014-5793(93)81331-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fourier transform infrared spectroscopy is used to estimate the secondary structure of bacteriorhodopsin dissolved in chloroform-methanol (1:1 v/v), 0.1 M LiClO4. Curve-fitting of the deconvolved spectra in the amide I region shows that the total content of alpha-helices, reverse turns and beta-sheets are similar to the native state. However, the alpha(II)-helices, which are the major helical class in native bacteriorhodopsin, are greatly decreased in the solubilized sample. Similarly, the reverse turns and the beta-sheets are strongly altered.

引用

页码：77 / 79

页数：3

共 22 条

[1] F-19 NMR-STUDY OF 5-FLUOROTRYPTOPHAN-LABELED BACTERIORHODOPSIN
ARSENIEV, AS
KURYATOV, AB
TSETLIN, VI
BYSTROV, VF
IVANOV, VT
OVCHINNIKOV, YA
[J]. FEBS LETTERS, 1987, 213 (02) : 283 - 288
[2] SEQUENCE-SPECIFIC H-1-NMR ASSIGNMENT AND CONFORMATION OF PROTEOLYTIC FRAGMENT 163-231 OF BACTERIOOPSIN
BARSUKOV, IL
ABDULAEVA, GV
ARSENIEV, AS
BYSTROV, VF
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 192 (02): : 321 - 327
[3] BARSUKOV IL, 1992, EUR J BIOCHEM, V206, P66
[4] EXAMINATION OF THE SECONDARY STRUCTURE OF PROTEINS BY DECONVOLVED FTIR SPECTRA
BYLER, DM
SUSI, H
[J]. BIOPOLYMERS, 1986, 25 (03) : 469 - 487
[5] CLADERA J, 1992, IN PRESS BIOCHEMISTR
[6] THE 5 RETINAL-PROTEIN PIGMENTS OF HALOBACTERIA - BACTERIORHODOPSIN, HALORHODOPSIN, P-565, P-370, AND SLOW-CYCLING RHODOPSIN
DENCHER, NA
[J]. PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1983, 38 (06) : 753 - 767
[7] PROTEIN SECONDARY STRUCTURES IN WATER FROM 2ND-DERIVATIVE AMIDE-I INFRARED-SPECTRA
DONG, A
HUANG, P
CAUGHEY, WS
[J]. BIOCHEMISTRY, 1990, 29 (13) : 3303 - 3308
[8] DETERMINATION OF THE SECONDARY STRUCTURE-CONTENT OF PROTEINS IN AQUEOUS-SOLUTIONS FROM THEIR AMIDE-I AND AMIDE-II INFRARED BANDS - COMPARISON BETWEEN CLASSICAL AND PARTIAL LEAST-SQUARES METHODS
DOUSSEAU, F
PEZOLET, M
[J]. BIOCHEMISTRY, 1990, 29 (37) : 8771 - 8779
[9] MODEL FOR THE STRUCTURE OF BACTERIORHODOPSIN BASED ON HIGH-RESOLUTION ELECTRON CRYOMICROSCOPY
HENDERSON, R
BALDWIN, JM
CESKA, TA
ZEMLIN, F
BECKMANN, E
DOWNING, KH
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1990, 213 (04) : 899 - 929
[10] MAGNETIC-RESONANCE OF MEMBRANES
KNOWLES, PF
MARSH, D
[J]. BIOCHEMICAL JOURNAL, 1991, 274 : 625 - 641

← 1 2 3 →