QUANTITATIVE BETA-GLUCURONIDASE ASSAY IN TRANSGENIC PLANTS

被引：12
|
作者：
VITHA, S
BENES, K
MICHALOVA, M
ONDREJ, M
机构：
[1] Institute of Plant Molecular Biology, Academy of Science of the Czech Republic, České Budějovice
来源：
BIOLOGIA PLANTARUM | 1993年 / 35卷 / 01期
关键词：
D O I：
10.1007/BF02921141
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Several factors influencing reliability of the quantitative fluorimetric beta-glucuronidase (GUS) assay in transgenic plant tissue have been investigated. We obtained linear dependence of fluorescence on both the duration of hydrolysis and the extract concentration. The stability of the enzyme in the homogenate was fairly high, the same as the stability of the substrate solution and of the final reaction product. The modification of the extraction/incubation buffer was proposed, resulting in several times higher activity in comparison with original procedure.
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页码：151 / 155
页数：5
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