ISOLATION AND SCREENING OF BASIDIOMYCETES WITH HIGH PEROXIDATIVE ACTIVITY

被引：89
作者：
DEJONG, E
DEVRIES, FP
FIELD, JA
VANDERZWAN, RP
DEBONT, JAM
机构：
[1] AGROTECHNOL RES INST ATO DLO, 6700 AA WAGENINGEN, NETHERLANDS
[2] AGR UNIV WAGENINGEN, DEPT FORESTRY, 6703 BV WAGENINGEN, NETHERLANDS
来源：
MYCOLOGICAL RESEARCH | 1992年 / 96卷
关键词：
D O I：
10.1016/S0953-7562(09)80121-4
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Sixty-seven Poly R-478 decolorizing basidiomycetes were isolated with a selective medium (containing hemp (Cannabis sativa) stem wood, guaiacol and benomyl). Several of the new isolates seemed to be promising manganese peroxidase-containing white-rot fungi. Enzyme assays indicated that either glyoxal or veratryl alcohol oxidase were present in the culture fluids of peroxidative strains. In contrast, lignin peroxidase was only detected in Phanerochaete chrysosporimm, despite attempts to induce this enzyme in other strains with oxygen and oxygen/veratryl alcohol additions. A highly significant correlation was found between two ligninolytic indicators: ethene formation from alpha-keto-gamma-methylthiolbutyric acid and the decolorization of a polymeric dye, Poly R. Three of the new isolates had significantly higher Poly R decolorizing activities compared to P. chrysosporium. The Poly R decolorization rate is a good assay when trying to optimize culture conditions for peroxidase/H2O2 production.
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页码：1098 / 1104
页数：7
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