CONSTRUCTION AND CHARACTERIZATION OF RADIATION HYBRIDS FOR CHROMOSOME-9, AND THEIR USE IN MAPPING COSMID PROBES ON THE CHROMOSOME

被引：16

作者：

JACKSON, CL ^{[1
]}

BRITT, DE ^{[1
]}

GRAW, SL ^{[1
]}

POTTS, A ^{[1
]}

SANTORO, K ^{[1
]}

BUCKLER, AJ ^{[1
]}

HOUSMAN, DE ^{[1
]}

MARK, HFL ^{[1
]}

机构：

[1] MIT,CTR CANC,CAMBRIDGE,MA 02139

来源：

SOMATIC CELL AND MOLECULAR GENETICS | 1992年 / 18卷 / 03期

关键词：

D O I：

10.1007/BF01233864

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Radiation hybrids were produced from a monochromosomal microcell hybrid (PK87-9) which contains only human chromosome 9 with an inserted marker on 9p. Doses of radiation ranging from 1000 to 8000 rads were used to produce a series of hybrids with different size fragments of human chromosome 9. The inserted dominant selectable marker was used to select for hybrids that preferentially maintain fragments of 9p. A panel of 53 radiation hybrids were characterized for 17 chromosome 9 markers. In addition, 17 hybrids were analyzed by fluorescent in situ hybridization (FISH). Hybrids were produced with breaks on both 9p and 9q, many of which appear to contain a single fragment of human chromosome 9. These hybrid cell lines were used to regionally localize 31 cosmids isolated from a chromosome 9 cosmid library. Six cosmids were mapped to intervals on 9p, six cosmids mapped to the centromeric region of the chromosome, and 19 mapped to 9q.

引用

页码：285 / 301

页数：17

共 41 条

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