ANALYSIS OF ENTEROCOCCUS-FAECALIS ISOLATES FROM INTERCONTINENTAL SOURCES BY MULTILOCUS ENZYME ELECTROPHORESIS AND PULSED-FIELD GEL-ELECTROPHORESIS

被引：45
作者：
TOMAYKO, JF
MURRAY, BE
机构：
[1] UNIV TEXAS,SCH MED,DEPT INTERNAL MED,HOUSTON,TX
[2] UNIV TEXAS,SCH MED,DEPT MICROBIOL & MOLEC GENET,HOUSTON,TX
[3] UNIV TEXAS,SCH MED,CTR INFECT DIS,HOUSTON,TX 77030
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 11期
关键词：
D O I：
10.1128/JCM.33.11.2903-2907.1995
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Pulsed-field gel electrophoresis (PFGE) and multilocus enzyme electrophoresis (MLEE) were compared in this study of 65 Enterococcus faecalis isolates recovered over a 20-year period from diverse geographic sources. Clonal relationships recognized by PFGE were also recognized by MLEE; however, MLEE recognized a greater number of isolates as belonging to clonal groups than did PFGE. Both techniques were reproducible and discriminatory, but MLEE more readily recognized relationships among large numbers of isolates. MLEE confirmed the previously reported clonal spread of beta-lactamase-producing E. faecalis to six hospitals in five states. MLEE provided a useful population framework of the E. faecalis isolates in this sample, while PPGE was able to differentiate among isolates within some MLEE clonal groups.
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页码：2903 / 2907
页数：5
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