QUANTITATIVE ESTIMATION OF CYTOCHROME-OXIDASE ACTIVITY AS DETERMINED BY STEREOLOGICAL MORPHOMETRY

被引：0

作者：

IWANO, M

WAKIYA, S

TSUCHITANI, N

NEGITA, G

YOKOMURA, EI

机构：

[1] Department of Biology, Faculty of Science, Nara Women's University, Nara, Kitauoya Nishimachi

来源：

ACTA HISTOCHEMICA ET CYTOCHEMICA | 1994年 / 27卷 / 02期

关键词：

D O I：

10.1267/ahc.27.153

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Cyanobacteria are photosynthetic prokaryotes. They have thylakoidal membrane that carry out oxygenic photosynthesis. They grow autotrophically in a general way. Nostoc muscorum M-14, a species of cyanobacteria, show exceptionally heterotrophic growth. DAB-histochemistry proved occurrence of cytochrome oxidase activity in thylakoidal membrane. Activity of cytochrome oxidase was quantitatively estimated by stereological morphometry based on modified Weibel's method. Activity of cytochrome oxidase increased by 3.5 times in heterotrophic cells compared with autotrophic cells. The enhancement was coincident with the increment of oxygen consumption measured with oxygen electrode. Quantitative DAB-histochemistry elucidated that heterotrophic condition modulates respiratory activity in the thylakoidal membrane of cyanobacteria. Presence of respiratory activity in photosynthetic membrane of cyanobacteria suggests evolutionary ancient nature of the membrane.

引用

页码：153 / 157

页数：5

共 12 条

[1] PHOTOOXIDATION OF 3,3'-DIAMINOBENZIDINE BY BLUE-GREEN-ALGAE AND CHLAMYDOMONAS-REINHARDII [J].