LOW-LEVEL CO2 LASER-INDUCED RELEASE OF CHROMIUM-51 FROM CANINE 2C5 GLIOSARCOMA CELLS

Recently, interest has grown in the area of low-power laser effects upon tissues. We used a Cr-51 cell labeling technique with glioma tissue to better understand these effects. Canine 2C5 gliosarcoma cells with intracellular Cr-51 were exposed to CO2 laser in the range of 0.2 to 3.0 j/CM2. Correlative analysis of the data indicated that there is a strong direct relationship between laser fluence and the percent of total intracellular Cr-51 released from the glioma cells with a coefficient of correlation (r) of +0.93. The calculated standard error of the correlation coefficient was +/- 0.06 and the coefficient of determination (r2) was 0.86. These results indicate that the Cr-51 cell labeling technique is a useful method for quantifying the low-power laser effects on the integrity of the cell membrane of gliosarcoma cells in vitro. However, further investigation is needed to clarify the specific mechanisms by which the CO2 laser induces changes upon these cells. (C) 1993 Wiley-Liss, Inc.