MOLECULAR-CLONING AND EXPRESSION OF THE GENE ENCODING HUMAN ANGIOTENSIN-II TYPE-2 RECEPTOR

The gene of human angiotensin II type 2 (AT(2)) receptor was isolated from a genomic DNA library prepared from human placenta. The coding region of the human AT(2) receptor gene was contained in a single exon coding segment of the gene indicating an intronless structure of the coding region. The amino acid sequence of human AT(2) receptor deduced from its nucleotide sequence has 363 amino acids and shows a high degree of sequence identity to rat and mouse receptor sequences. Specific binding of [I-125]Sar(1)Ile(8)-angiotensin II was demonstrated in COS-7 cells transfected with a plasmid containing the human AT(2) sequence. Scatchard analysis and ligand displacement profile were typical of the AT(2) receptor. Reverse transcription-polymerase chain reaction analysis showed that AT(2) receptor mRNA was expressed in adult uterus and pheochromocytoma. (C) 1994 Academic Press, Inc.