CLONING OF DNA FROM A RHODOCOCCUS STRAIN CONFERRING THE ABILITY TO DECOLORIZE SULFONATED AZO DYES

被引：100

作者：

HEISS, GS ^{[1
]}

GOWAN, B ^{[1
]}

DABBS, ER ^{[1
]}

机构：

[1] UNIV WITWATERSRAND,DEPT GENET,PRIVATE BAG 3,PO WTIS 2050,JOHANNESBURG 2001,SOUTH AFRICA

来源：

FEMS MICROBIOLOGY LETTERS | 1992年 / 99卷 / 2-3期

关键词：

AZO DYE DECOLORIZATION; DEGRADATION OF XENOBIOTICS; DNA CLONING; RHODOCOCCUS; NOCARDIOFORMS;

D O I：

10.1111/j.1574-6968.1992.tb05571.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Azo dyes are recalcitrant pollutants. Two sulfonated azo dyes, Orange II and Amido black, are effectively decolorized by certain nocardioform strains of the genus Rhodococcus. A mutant of one of these strains was isolated which had lost azo-dye decolorizing ability and the strain was used to clone DNA conferring this ability, by screening a BclI library constructed from DNA of a decolorizing strain. The relevant genetic information was located on a 6.3-kb fragment of DNA.

引用

页码：221 / 226

页数：6

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