EFFECT OF FLASH PHOTOREACTIVATION ON ESCHERICHIA-COLI RECA INDUCTION BY ULTRAVIOLET-LIGHT

被引：0

作者：

YAMAMOTO, K

机构：

来源：

FEMS MICROBIOLOGY LETTERS | 1992年 / 99卷 / 01期

关键词：

PHOTOREACTIVATION; RECA INDUCTION; MUTAGENESIS; ULTRAVIOLET LIGHT; ESCHERICHIA-COLI;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Excision-deficient Escherichia coli, carrying the gene for the photolyase on a multicopy plasmid, were irradiated with ultraviolet (UV) light then photoreactivated by illumination delivered from a camera flash unit. Such instantaneous illumination monomerizes only cyclobutane pyrimidine dimers already bound by the photolyase. Whereas the lethal effect of UV light and the number of C-to-T transition-type mutations induced by UV irradiation were both significantly reduced by subsequent irradiation with a single flash of light, single-flash photoreactivation did not reverse the induction of the recA gene by UV light. The results indicate, therefore, that non-photoreactivable DNA lesions play a role in recA induction.

引用

页码：31 / 36

页数：6

共 16 条

[1] SPECIFICITY OF MUTATION BY UV-LIGHT AND DELAYED PHOTOREVERSAL IN UMUC-DEFECTIVE ESCHERICHIA-COLI-K-12 - A TARGETING INTERMEDIATE AT PYRIMIDINE DIMERS [J].