EFFECTS OF INHIBITORS OF LIPID-SYNTHESIS ON REPLICATION OF ROUS-SARCOMA VIRUS - SPECIFIC EFFECT OF CERULENIN ON PROCESSING OF MAJOR NON-GLYCOSYLATED VIRAL STRUCTURAL PROTEINS

被引：21

作者：

GOLDFINE, H ^{[1
]}

HARLEY, JB ^{[1
]}

WYKE, JA ^{[1
]}

机构：

[1] IMPERIAL CANC RES FUND LABS,DEPT TUMOUR VIROL,LINCOLNS INN FIELDS,LONDON WC2,ENGLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1978年 / 512卷 / 02期

关键词：

D O I：

10.1016/0005-2736(78)90249-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of two inhibitors of lipid biosynthesis on the replication of Rous sarcoma virus Prague C strain in chick embryo fibroblasts have been examined in media containing delipidated serum. 25-Hydroxycholesterol, which markedly inhibits the incorporation of [1-14C]acetate into sterols, had no effect on the formation of infectious virions or on the synthesis and processing of intracellular virion proteins. Cerulenin strongly inhibited [1-14C]acetate incorporation into fatty acids and partially inhibited its incorporation into sterols in chick embryo cells. Rous sarcoma virus production as measured by focus formation and by the production of [35S]methionine-labeled virions was strongly inhibited within 5 h after cerulenin addition to infected cultures. Examination of extracts of these cells revealed the accumulation of the 76 000 dalton precursor (Pr76) of the major non-glycosylated virion structural proteins, p27, p19, p15 and p12. The failure to process the 76 000 dalton precursor was coincident in time with the decrease in viron production. Neither whole serum nor mixtures of fatty acids plus cholesterol were able to reverse the effects of cerulenin. © 1978.

引用

页码：229 / 240

页数：12

共 23 条

[11]

KANDUTSCH AA, 1974, J BIOL CHEM, V249, P6057

[12]

KANDUTSCH AA, 1977, J BIOL CHEM, V252, P409

[13] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[14] VIRAL GLYCOPROTEIN SYNTHESIS UNDER CONDITIONS OF GLUCOSAMINE BLOCK IN CELLS TRANSFORMED BY AVIAN-SARCOMA VIRUSES [J].

LEWANDOWSKI, LJ ;

SMITH, RE ;

BOLOGNESI, DP ;

HALPERN, MS .

VIROLOGY, 1975, 66 (02) :347-355

[15] ANTIBIOTIC CERULENIN, A NOVEL TOOL FOR BIOCHEMISTRY AS AN INHIBITOR OF FATTY-ACID SYNTHESIS [J].

OMURA, S .

BACTERIOLOGICAL REVIEWS, 1976, 40 (03) :681-697

[16] ISOLATION AND CHARACTERIZATION OF PLASMA MEMBRANE FROM CULTURED CELLS .1. CHEMICAL COMPOSITION OF MEMBRANE ISOLATED FROM UNINFECTED AND ONCOGENIC RNA VIRUS-CONVERTED CHICK EMBRYO FIBROBLASTS [J].

PERDUE, JF ;

KLETZIEN, R ;

MILLER, K .

BIOCHIMICA ET BIOPHYSICA ACTA, 1971, 249 (02) :419-+

[17] PHOSPHOLIPID COMPOSITION OF ROUS SARCOMA VIRUS, HOST CELL MEMBRANES AND OTHER ENVELOPED RNA VIRUSES [J].

QUIGLEY, JP ;

RIFKIN, DB ;

REICH, E .

VIROLOGY, 1971, 46 (01) :106-+

[18] A VIRUS IN CHICK EMBRYOS WHICH INDUCES RESISTANCE IN VITRO TO INFECTION WITH ROUS SARCOMA VIRUS [J].

RUBIN, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1960, 46 (08) :1105-1119

[19] ANALYSIS OF BACTERIOPHAGE T7 EARLY RNAS AND PROTEINS ON SLAB GELS [J].

STUDIER, FW .

JOURNAL OF MOLECULAR BIOLOGY, 1973, 79 (02) :237-+

[20]

Vogt PK, 1969, FUNDAMENTAL TECHNIQU, P198

← 1 2 3 →