STEREOSELECTIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY WITH FLUOROMETRIC DETECTION FOR THE ISOMERS OF MIVACURIUM IN HUMAN PLASMA

被引：16

作者：

BROWN, AR ^{[1
]}

JAMES, CD ^{[1
]}

WELCH, RM ^{[1
]}

HARRELSON, JC ^{[1
]}

机构：

[1] BURROUGHS WELLCOME CO,DIV PHARMACOKINET & DRUG METAB,3030 CORNWALLIS RD,RES TRIANGLE PK,NC 27709

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 578卷 / 02期

关键词：

D O I：

10.1016/0378-4347(92)80430-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic assay with fluorometric detection was developed for the analysis of the stereoisomers of mivacurium, a new short-acting neuromuscular blocker, in plasma. The isomers were isolated form plasma by solid-phase extraction with C18 and anion-exchange cartridges. The extracts were chromatographed on a LiChrosphere 60 RP Select B column (125 mm x 4.6 mm I.D.) using a mobile phase of acetonitrile-water (4:6, v/v) containing 0.005 M octanesulfonic acid. The fluorescence excitation and emission wavelengths were 202 and 320 nm, respectively. The accuracy and precision of the assay, expressed as the percentage deviation of measured values from true values and the percentage coefficient of variation, respectively, were less-than-or-equal-to 10% at all concentrations except for the percentage coefficient of variation at the lower limit of quantitation (5 ng/ml). The assay has been successfully used for the analysis of plasma samples from a pharmacokinetic study in human volunteers.

引用

页码：302 / 308

页数：7