MOLECULAR-CLONING AND DEVELOPMENTAL EXPRESSION OF A HUMAN KIDNEY S-ADENOSYLMETHIONINE SYNTHETASE

被引：75
作者：
HORIKAWA, S
TSUKADA, K
机构：
[1] Department of Pathological Biochemistry, Medical Research Institute, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo, 101, Kanda-surugadai
来源：
FEBS LETTERS | 1992年 / 312卷 / 01期
关键词：
S-ADENOSYLMETHIONINE; ISOZYME; CDNA SEQUENCE; HUMAN KIDNEY;
D O I：
10.1016/0014-5793(92)81405-B
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
cDNA clones encoding the human kidney S-adenosylmethionine synthetase (kidney-type isozyme) were isolated. The amino acid sequence deduced from the cDNA indicates that this enzyme contains 395 amino acids and has a molecular mass of 43,660 Da. The predicted amino acid sequence of this protein shares 84% similarity with that of human liver S-adenosylmethionine synthetase (liver-type isozyme). In addition, the developmental expression of these two isozyme mRNAs has been studied in the human liver using the reverse transcription-polymerase chain reaction (RT-PCR).
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页码：37 / 41
页数：5
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