KINETIC-STUDIES ON RECOMBINANT HUMAN RENIN WITH RECOMBINANT HUMAN ANGIOTENSINOGEN DERIVED FROM CHINESE-HAMSTER OVARY CELLS

被引：4

作者：

HATAE, T

TAKIMOTO, E

FUKAMIZU, A

MURAKAMI, K

机构：

[1] Institute of Applied Biochemistry, University of Tsukuba, Tsukuba

来源：

BIOMEDICAL RESEARCH-TOKYO | 1992年 / 13卷 / 05期

关键词：

D O I：

10.2220/biomedres.13.381

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The reaction kinetics of recombinant human renin (rh-renin) derived from Chinese hamster ovary cells reacting with recombinant human angiotensinogen (rhANG) has been shown to be similar to those of human renal renin with human plasma angiotensinogen. At pH 6.5 and 37-degrees-C, the Michaelis constant (Km) of rh-renin reacting with rhANG was 1.62 muM, and catalytic center activity (kcat) was 1.05 per second. In this study, we provided a basis for the study of the structure-function relationships of the human renin-angiotensin system using recombinant proteins.

引用

页码：381 / 383

页数：3

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