CELL-FREE BIOSYNTHESIS AND SOURCE OF HYDROXYL-GROUPS IN (12R,13S)-DIHYDROXY-(5Z,8Z,10E,14Z)-EICOSATETRAENOIC ACID, A NOVEL EICOSANOID FROM THE MARINE ALGA GRACILARIOPSIS-LEMANEIFORMIS

A cell-free biosynthetic study with the marine macrophyte Gracilariopsis lemaneiformis showed by mass spectrometry and C-13 nmr that molecular oxygen was the source of the two hydroxyl groups in the novel eicosanoid (12R,13S)-dihydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoic aicd [3].