METABOLIC ASPECTS OF THE GROWTH OF A PINK-PIGMENTED FACULTATIVE METHYLOTROPH IN CARBON-LIMITED CHEMOSTAT CULTURE

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作者
GIRIO, FM [1 ]
ATTWOOD, MM [1 ]
机构
[1] UNIV SHEFFIELD,DEPT MOLEC BIOL & BIOTECHNOL,SHEFFIELD S10 2TN,S YORKSHIRE,ENGLAND
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中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The regulation of carbon metabolism in a pink-pigmented facultative methylotroph has been studied. In methanol-limited chemostat culture a pH optimum at 7.0 with a narrow growth rate optimum with respect to growth yield and metabolic uncoupling was revealed. The average growth yield was 14 +/- 0.036 g.mol-1 and the organism displayed a low maintenance energy and high maximum specific growth rate. When the carbon concentration in the feed remained constant and the dilution rate increased a deviation from linearity between substrate consumption and growth rate was found at higher growth rates. The addition of a pulse of methanol to a carbon-limited culture showed that anabolism could be dissociated from catabolism with the resulting accumulation of formaldehyde in concentrations which were not lethal.
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页码:77 / 82
页数:6
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