NOVEL NARROW-HOST-RANGE VECTORS FOR DIRECT CLONING OF FOREIGN DNA IN PSEUDOMONAS

被引：3

作者：

BOIVIN, R

BELLEMARE, G

DION, P

机构：

[1] UNIV LAVAL, FAC AGR & FOOD SCI, DEPT PHYTOL, QUEBEC CITY G1K 7P4, QUEBEC, CANADA

[2] UNIV LAVAL, FAC SCI & ENGN, DEPT BIOCHEM, QUEBEC CITY G1K 7P4, QUEBEC, CANADA

来源：

CURRENT MICROBIOLOGY | 1994年 / 28卷 / 01期

关键词：

D O I：

10.1007/BF01575984

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Narrow-host-range vectors, based on an indigenous replicon and containing a multiple cloning site, have been constructed in a Pseudomonas host capable of growth on unusual substrates. The new cloning vectors yield sufficient amounts of DNA for preparative purposes and belong to an incompatibility group different from that of the incP and incQ broad-host-range vectors. One of these vectors, named pDB47F, was used to clone, directly in Pseudomonas, DNA fragments from Agrobacterium, Pseudomonas, and Rhizobium. A clone containing Agrobacterium and Km(R) gene sequences was transformed with a higher efficiency than an RSF1010-derived vector (by as much as 1250-fold) in four out of five Pseudomonas strains tested. The considerable efficiency obtained with this system makes possible the direct cloning and phenotypic selection of foreign DNA in Pseudomonas.

引用

页码：41 / 47

页数：7

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