TEMPERATURE AS A VARIABLE IN REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATIONS OF PEPTIDE AND PROTEIN SAMPLES .1. OPTIMIZING THE SEPARATION OF A GROWTH-HORMONE TRYPTIC DIGEST

被引：103
作者：
HANCOCK, WS
CHLOUPEK, RC
KIRKLAND, JJ
SNYDER, LR
机构：
[1] LC RESOURCES INC,ORINDA,CA 94563
[2] ROCKLAND TECHNOL INC,NEWPORT,DE 19804
[3] GENENTECH INC,S SAN FRANCISCO,CA 94080
来源：
JOURNAL OF CHROMATOGRAPHY A | 1994年 / 686卷 / 01期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1016/0021-9673(94)00077-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Peptide and protein samples are often complex mixtures that contain a number of individual compounds. The initial HPLC separation of such samples typically results in the poor resolution of one or more band pairs. Various means have been suggested for varying separation selectivity so as to minimize this problem. In this study of a tryptic digest of recombinant human growth hormone, the simultaneous variation of temperature and gradient ;steepness was found to be a convenient and effective means of varying selectivity and optimizing the separation. The use of computer simulation greatly facilitated this investigation.
引用
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页码：31 / 43
页数：13
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