INSITU HYBRIDIZATION AS A METHODOLOGICAL TOOL FOR THE NEUROSCIENTIST

被引：45
作者：
EMSON, PC
机构：
[1] MRC Group, Dept of Neurobiology, AFRC, Cambridge, CB2 4AT, Babraham
来源：
TRENDS IN NEUROSCIENCES | 1993年 / 16卷 / 01期
关键词：
D O I：
10.1016/0166-2236(93)90041-J
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The technique of in-situ hybridization is now well established for the identification and localization of both DNA and mRNA in cells in the nervous system. For the nonspecialist neuroscientist, use of the technique has been greatly facilitated by the availability of convenient commercial kits for producing isotopically labelled cDNA and cRNA probes. Additionally, the development of synthetic oligonucleotide probes and nonradioactive detection for DNA or RNA has greatly contributed to accessibility of the technique.
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页码：9 / 16
页数：8
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