EFFECTS OF PURIFIED SNAKE-VENOM TOXIC COMPONENTS ON SUBSTANCE-P BINDING TO RAT-BRAIN MEMBRANES

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UTKIN, YN
KASHEVEROV, IE
TSETLIN, VI
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BIOORGANICHESKAYA KHIMIYA | 1992年 / 18卷 / 05期
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Ion-exchange HPLC is used for purification of the snake venom alpha-neurotoxin, kappa-bungarotoxin, cytotoxins, and phospholipases A2. Among these purified polypeptides, phospholipases As are found to be the most potent in inhibiting the substance P binding to rat brain membranes, K1 approximately 10(-8) M. Other toxins are weak inhibitors (K1 greater-than-or-equal-to 10(-4)-10(-5) M), earlier data on the inhibiting activity of alpha-bungarotoxin being caused by the commercial preparations' contamination with phospholipase A2.
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页码：635 / 639
页数：5
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